<b>Efficient nursery production and multiple-shoot clumps formation from shoot tiller-derived shoot apices of dwarf napiergrass (Pennisetum purpur</b><b>eum Schumach. ) </b>

DOI
  • UMAMI Nafiatul
    Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki, Miyazaki 889-2192, Japan Faculty of Animal Science, Gadjah Mada University, Yogyakarta 55281, Indonesia
  • GONDO Takahiro
    Frontier Science Research Center, University of Miyazaki, Miyazaki 889-2192, Japan
  • ISHIGAKI Genki
    Frontier Science Research Center, University of Miyazaki, Miyazaki 889-2192, Japan
  • RAHMAN Mohammad Mijanur
    Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
  • AKASHI Ryo
    Frontier Science Research Center, University of Miyazaki, Miyazaki 889-2192, Japan

Bibliographic Information

Other Title
  • わい性ネピアグラスにおける腋芽生長点からの多芽体形成と効率的な苗生産

Abstract

<tt>The objective of this study was to improve an </tt><tt>in vitro </tt><tt>tissue culture system for the dwarf napiergrass (</tt><tt>Pennisetum purpureum </tt><tt>Schumach. ). Additionally, it was needed special treatments for acclimatization, and carried the risks of somaclonal variation and physiological aberrations. Shoot apices as initial explants were isolated aseptically from shoottillers, and cultured on </tt><tt>in vitro </tt><tt>MS (Murashige and Skoog, 1962) medium containing 3.0% sucrose and 0.3% phytagel. The most effective phytohormone treatment for multiple-shoot clumps (MSCs) induction was 0.1 mg L</tt><tt>-1 </tt><tt>2,4-D plus 2.0 mg L</tt><tt>-1 </tt><tt>BAP. The addition of 50 </tt>μ<tt>M CuSO4 could increase the percentage of MSCs proliferation. Plant regeneration frequency was achieved up to 84% by culturing the MSCs on solid MS medium containing 0.1 mg L</tt><tt>-1 </tt><tt>NAA and 2.0 mg L</tt><tt>-1 </tt><tt>BAP. All regenerants were successfully grown up in the soil. Compared to control plants, </tt><tt>in vitro </tt><tt>regenerated plants did not reveal any significant difference (</tt><tt>P </tt><tt>> 0.05) on morphological characteristics and DNA content. The results of this study suggest that improved protocols for </tt><tt>in vitro </tt><tt>propagation would provide high quality nursery plant production of dwarf napiergrass in </tt><tt>grassland field. </tt>

Journal

Keywords

Details 詳細情報について

  • CRID
    1390282680320476416
  • NII Article ID
    130004555651
  • DOI
    10.11461/jwaras.55.121
  • ISSN
    21851670
    2185081X
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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