ON THE BIOLOGY OF INCLUSION BODIES IN VIRUS DISEASES

A further observation of inclusion bodies in monocytes drawn out from the peritoneal cavity at varied intervals after intraperitoneal inoculation of ectromelia virus was made availing phase contrast microscope. A. 24 hours after the inoculation, young inclusion bodies appeared distinctly as round or oval ones in slightly deeper tone than the surrounding cytoplasm. Elementary bodies with the definite size were not distributed inside these inclusion bodies. This fact shows that in early stage two components of the inclusion body, namely, the matrix and elementary bodies develop from different bases, the former from cellular constituent, while the latter only remaing on the surface of this matrix. In the following stages, elementary bodies begin to invade the matrix membrane and subsequently fill up the inclusion body.<br>B. From this new finding, we were obliged to seek the initial structure for these inclusion bodies. As the young inclusion bodies located in general amidst the mitochondrial zone more or less at the periphery of the cytoplasm, indifferent from the Golgi zone, so we set focus to the relationship between the initial inclusion body and mitochondrias. The initial form found 5 to 6 hours after the virus inoculation starts from the thickening of one or several agglomerated mitochondrias, which develop after 10 to 12 hours as a distinct form of the inclusion body.<br>Observing the various modi of infected tissues, we can classify two types of the cellular infection; one, like liver cells, being damaged till to rapid necrosis without formation of inclusion body in general, whereas the other for instance monocytes and epidermis, being relative resistant with the formation of the inclusion body in the same animal. The auther is tempted to consider the inclusion as a buffer structure developed from the cellular organella against virus.