PURIFICATION AND CONCENTRATION OF GD VII STRAIN OF MOUSE ENCEPHALOMYELITIS VIRUS

DOI

Bibliographic Information

Other Title
  • マウス腦脊髓炎ウイルスGD VII株の精製実験に関する研究 (1)
  • I. PURIFICATION AND CONCENTRATION OF GD VII VIRUS BY MEANS OF ETHER EXTRACTION, HEMAGGLUTINATION, AND METHANOL AND ACETONE PRECIPITATION
  • エーテル抽出・血球吸着誘出及びメタノール・アセトン沈澱法による実験

Description

The experiments on the purification and concentration of GD VII virus by hemagglutination have been reported already. This purification method of the virus by means of adsorption to and elution from erythrocytes is a simple and effective procedure from the results obtained, however a good yield of virus is not acquired in an attempt to concentrate the virus by the procedure because of hemolysis.<br>Therefore, methanol and acetone precipitation methods were employed after elution of virus from blood cells.<br>The results obtained indicate that the hemagglutination titer of the final concentrated preparation was 163, 840 compared with the titer of the original material 1280, and approximately 99.3% of the non-viral protein was removed. In other words, while the original meterial contained. 1×103.4 hemagglutinating units per mg N, the purified material contained 1×107.1. The best conditions for addition of methanol and acetone to virus suspension were as follows: final concentration of methanol was 30%, the mixture of methanol and virus materials was kept for 6 hours in the cold, and as the eluant for virus from the methanol precipitate the distilled water was employed, and the final concentration of acetone was 50%, the mixture was shaken for 30 minutes, centrifuged, and the eluant from acetone precipitate was the distilled water similarly to the case of methanol.

Journal

  • VIRUS

    VIRUS 5 (4), 271-277, 1955

    The Japanese Society for Virology

Details 詳細情報について

  • CRID
    1390282680320813184
  • NII Article ID
    130003854275
  • DOI
    10.2222/jsv1951.5.271
  • ISSN
    18843425
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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