A super photostable fluorescent dye that survives in repeated STED laser irradiation

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  • Sato Yoshikatsu
    Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya Univ.
  • Wang Chenguang
    Department of Chemistry, Graduate School of Science, Nagoya Univ.
  • Fukazawa Aiko
    Department of Chemistry, Graduate School of Science, Nagoya Univ.
  • Taki Masayasu
    Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya Univ. Department of Chemistry, Graduate School of Science, Nagoya Univ.
  • Higashiyama Tetsuya
    Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya Univ. Division of Biological Science, Graduate School of Science, Nagoya Univ. JST, ERATO, Higashiyama Live-Holonics Project, Nagoya Univ.
  • Yamaguchi Shigehiro
    Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya Univ. Department of Chemistry, Graduate School of Science, Nagoya Univ.

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Other Title
  • 超耐光性蛍光色素が拓く蛍光バイオイメージング
  • チョウタイコウセイ ケイコウ シキソ ガ ヒラク ケイコウ バイオイメージング

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Description

Super resolution microscopy enables us to acquire images possessing much higher resolution than optical diffraction unit. Photostability of fluorescent dye is a definitely important property in super resolution microscopy, although required properties to fluorescence dye are varied and specialized dependent on the different super resolution technologies. Especially for STED imaging, exceptional photostability surviving in intense STED laser is required for repeated super resolution imaging and development of suitable fluorescence dye is awaited. Here, we introduce a phosphole oxide based fluorescent dye possessing unprecedented photostability sufficient for continuous STED imaging.

Journal

  • PLANT MORPHOLOGY

    PLANT MORPHOLOGY 28 (1), 9-13, 2016

    The Japanese Society of Plant Morphology

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