Gene expression analysis of Mysid Crustacea (<i>Americamysis bahia</i>) exposed to Triclosan and Triclocarban using DNA microarray

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  • Uchida Masaya
    Ecogenomics, Inc. Graduate School of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Miura Sonoko
    Ecogenomics, Inc. Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Hirano Masashi
    Center for Marine Environmental Studies (CMES), Ehime University
  • Inokuchi Ayako
    Graduate School of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Yamauchi Ryoko
    Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Yoshidu Remi
    Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Nakamura Hiroshi
    Ecogenomics, Inc.
  • Kagami Yoshihiro
    Ecogenomics, Inc.
  • Kusano Teruhiko
    Ecogenomics, Inc.
  • Koga Minoru
    Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • Arizono Koji
    Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto

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Other Title
  • アミDNAマイクロアレイを用いたトリクロサンおよびトリクロカルバンの生態影響評価
  • アミ DNA マイクロアレイ オ モチイタ トリクロサン オヨビ トリクロカルバン ノ セイタイ エイキョウ ヒョウカ

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Abstract

 Triclosan (TCS) and Trichlorocarban (TCC) are widely used as antibacterial agent in various industrial products,such as textile goods, soap, shampoo, liquid toothpaste and cosmetics, and often detected in wastewater effluent. The aim of this study was to investigate toxicologically significant effects of TCS and TCC exposure on gene expression in mysid (Americamysis bahia) using DNA microarray. Juvenile mysid were exposed to 0.5 µg/L of TCS and 0.05 µg/L of TCC for 24 hours, and mRNA expression profiles in their whole bodies were analyzed. We identified statistically significant (p<0.05) regulation (expression ratio of exposed to control > 3) in 312 genes responding to 0.5 µg/L of TCS and 264 genes responding to 0.05 µg/L of TCC. There were merely 231 genes found responding to both exposures of TCS and TCC. The up-regulation in response to TCS and TCC was observed in vitellogenin and clottable protein. These genes are expressed more than 30 times in both experimental plots, which indicates the applicability of them in serving as biomarkers of antibacterial agents in mysid. These results suggested that endocrine disrupting effect of TCS and TCC would be potent for marine crustacean.

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