Dietary lactosucrose suppresses influenza A (H1N1) virus infection in mice

  • KISHINO Eriko
    Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd., 1-1-1 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
  • TAKEMURA Naho
    Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd., 1-1-1 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
  • MASAKI Hisaharu
    Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd., 1-1-1 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
  • ITO Tetsuya
    Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd., 1-1-1 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
  • NAKAZAWA Masatoshi
    Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan

書誌事項

公開日
2015
DOI
  • 10.12938/bmfh.2015-005
公開者
BMFH出版会

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説明

This study examined the effects of lactosucrose (4G-β-D-galactosylsucrose) on influenza A virus infections in mice. First, the effects of lactosucrose on fermentation in the cecum and on immune function were investigated. In female BALB/c mice, lactosucrose supplementation for 6 weeks promoted cecal fermentation and increased both secretory IgA (SIgA) levels in feces and total IgA and IgG2a concentrations in serum. Both the percentage of CD4+ T cells in Peyer’s patches and the cytotoxic activity of splenic natural killer (NK) cells increased significantly in response to lactosucrose. Next, we examined the effects of lactosucrose on low-dose influenza A virus infection in mice. After 2 weeks of dietary supplementation with lactosucrose, the mice were infected with low-dose influenza A virus. At 7 days post infection, a comparison with control mice showed that weight loss was suppressed, as were viral titers in the lungs. In the spleens of lactosucrose-fed mice, there was an increase in the percentage of NK cells. Lastly, mice fed lactosucrose were challenged with a lethal dose of influenza A virus. The survival rate of these mice was significantly higher than that of mice fed a control diet. These results suggested that lactosucrose supplementation suppresses influenza A virus infection by augmenting innate immune responses and enhancing cellular and mucosal immunity.

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