Polymerase chain reaction-single strand conformational polymorphism analysis of intra- and interspecific variations in organellar DNA regions of Aegilops mutica and related species.

DOI Web Site Web Site PubMed 参考文献69件
  • Ohsako Takanori
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
  • Wang Gui-Zhi
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
  • Miyashita Naohiko T.
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University

書誌事項

タイトル別名
  • Polymerase chain reaction-single strand

この論文をさがす

抄録

In order to study the phylogeny of Aegilops mutica in the genera of Triticum and Aegilops, variations in chloroplast and mitochondrial DNA regions were investigated by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. Nine lines, each of Ae. mutica and Ae. speltoides, were studied together with nine other Triticum and Aegilops species, including T. aestivum. By analyzing 9.7-kb chloroplast and 13.1-kb mitochondrial DNA regions, a total of 268 bands were detected, of which 176 (65.7%) showed variation within and/or between species. The level of intraspecific variation of Ae. mutica was lower than that of Ae. speltoides. The low level of the intraspecific variation of Ae. mutica was contrary to the expectation from previous studies on morphological and cytolo-gical characters. In the phylogenetic trees based on SSCP, Ae. mutica, Ae. speltoides and the other four species of the section Sitopsis (the subsection Emarginata) were separated into three different clusters. In addition, T. aestivum was included in the cluster of Ae. speltoides in the phylogenetic trees. This result suggests that Ae. speltoides is the cytoplasmic donor of common wheat.<br>

収録刊行物

参考文献 (69)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ