Haploid plants regenerated from the pollen callus of wheat (Triticum aestivum L.).

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  • SHIMADA Takiko
    Laboratory of Genetics, Faculty of Agriculture, Kyoto University

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  • 普通小麦花粉由来カルスの半数体植物再分化〔英文〕
  • フツウ コムギ カフン ユライ カルス ノ ハンスウタイ ショクブツ サイ ブ

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Anthers of Triticum aestivum L, cv. Chinese Spring and of some Japanese and Chinese wheat cultivars were cultured on the N6 basal medium supplemented with inositol, glutamine, serine and 2, 4-D after the treatment with low temperature (5°C) for 7 days. Haploid calluses were obtained from the pollen of Chinese Spring, Orofen, Dansheng 15 and (Orofen×Hsiaoyen)F1, and were maintained by continuous subcultures, in which calluses were transferred once for every 30 to 40 days on the PM-64 basal medium supplemented with 2mg/l of 2, 4-D. Many regenerated plantlets were obtained on the RM-64 basal medium supplemented with 1mg/l of IAA and kinetin. The regenerated plants were mostly haploid and green. A few plants from Orofen and Dansheng 15 were diploid. The haploid calluses remained capability of differentiation to plantlets for about a year, and normal plants were restored from them. These haploid calluses from wheat pollen may be useful in mutant selection studies.

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