ヒト神経芽腫細胞におけるダブルマイニュートのエンドデュープリケーション誘導とその複製様式〔英文〕

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  • TAKAYAMA Susumu
    Biological Laboratory, School of Science, Kwansei Gakuin University
  • HIRANO Yukio
    Biological Laboratory, School of Science, Kwansei Gakuin University
  • HIRAMATSU Hisanao
    Biological Laboratory, School of Science, Kwansei Gakuin University

書誌事項

タイトル別名
  • Induction of endoreduplication in double minutes of the human neuroblastoma cells and the replication pattern.
  • ヒト シンケイガシュ サイボウ ニ オケル ダブル マイニュート ノ エンドデ

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説明

Induction of endoreduplication (ERD) using Hoechst 33258 as well as colcemid was carried out in cultured neuroblastoma (NB) line cells. In these endoreduplicated cells, the majority of double minutes (DMs) appeared to take a diplochromosome like configuration to form a cluster consisting of four minute elements, assuming a complex DM. Sister chromatid differential staining (SCD) using 5-bromo-2'-deoxyuridine (BrdUrd) revealed the non-random distribution of the stained chromatids among four chromatids composing each diplochromosome, suggesting the occurrence of so-called "outside replication" of DNA strands during the process of ERD. The same pattern of differential staining was also found in the quadruple minutes of each endoreduplicated DM. Since DMs are acentric, the present results suggest that centromeres do not play any essential role in the formation of diplochromosomes observed in the conventional cytologic preparations and that centromeres are probably not responsible for the phenomenon of the "outside replication" of DNA strands.<br>

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