Relative DNA content of individual chromosomes, karyotype and ploidy in crown gall tumor cells of Crepis capillaris.

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  • KODAMA Akira
    Department of Biology, Hiroshima Agricultural College, Saijo, Higashihiroshima 724 and Department of Botany, Ohio University
  • MITCHELL John P.
    Department of Biology, Hiroshima Agricultural College, Saijo, Higashihiroshima 724 and Department of Botany, Ohio University

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  • クレピスのクラウンゴール腫瘍細胞における染色体の相対DNA量,核型および倍数性〔英文〕
  • クレピス ノ クラウンゴール シュヨウ サイボウ ニ オケル センショクタイ

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Abstract

Levels of ploidy, karyotype, mitosis and relative DNA content of chromosomes in crown gall tumor cells in stem and leaf of Crepis capillaris were studied. Except for about 7% tetraploid mitoses, dividing cells were found to be mainly diploid in both stem and leaf tumors. The karyotype and mitosis in tumor cells were not detectably different from normal cells. The relative DNA content of homologous pairs of chromosomes in the genome of tumor cells was similar to that of root tip cells. These observations indicate that no dramatic change in DNA content or DNA distribution occurred when tissue was transformed to crown gall tumor.

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