大腸菌と枯草菌で共用できるシャトルベクタ--3-pAMα1由来のテトラサイクリン耐性遺伝子とpACYC177由来の複製開始領域のDNAの塩基配列〔英文〕
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- ISHIWA Hiromi
- Yakult Institute for Microbiological Research
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- SHIBAHARA Harue
- Yakult Institute for Microbiological Research
書誌事項
- タイトル別名
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- New shuttle vectors for Escherichia coli and Bacillus subtilis. III. Nucleotide sequence analysis of tetracycline resistance gene of pAM.ALPHA.1 and ori-177.
- ダイチョウキン ト コソウキン デ キョウヨウデキル シャトル ベクター 3
- New shuttle vectors for Escherichia coli and Bacillus subtilis. II. Nucleotide sequence analysis of tetracycline resistance gene pAMα1 and ori-177
- New shuttle vectors for Escherichia coli and Bacillus subtilis. III. Nucleotide sequence analysis of tetracycline resistance gene of pAMα1 and ori-177
- III. Nucleotide sequence analysis of tetracycline resistance gene of pAMα1 and <i>ori</i>-177
この論文をさがす
説明
A small plasmid pHY163PLK (2519bp) was constructed from the shuttle vector pHY300PLK. pHY163PLK contains the replication origin of pACYC-177, the tetracycline resistance gene (tetα1) of pAMα1 derived from Streptococcus faecalis and eight continuous unique cloning sites of a polylinker region (EcoRI, SmaI, BamHI, SalI, PstI, BglII, XbaI and HindIII). pHY163PLK, a copy-number mutant, has one base substitution in RNA I region compared with that of p15A which is the ancestor of ori-pHY163PLK. The tetα1, which codes 458 amino acids (1374bp), is highly homologous to the TcR genes of other Gram-positive bacteria but completely different from the TcR gene encoded by pBR322 in Gram-negative bacteria. The regulatory region of tetα1 (constitutive type) has an insertion of seven base pairs in the leader peptide region of a related inducible TcR gene. (key words: tetracycline resistance gene; pAMα1; Streptococcus; nucleotide sequence; amino acid sequence)
収録刊行物
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- 遺伝学雑誌
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遺伝学雑誌 60 (5), 485-498, 1985
日本遺伝学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282680421518720
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- NII論文ID
- 130000060523
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- NII書誌ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
- http://id.crossref.org/issn/0021504X
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- NDL書誌ID
- 3059151
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可