φ80追加感染阻害遺伝子(cor)のクローニング〔英文〕

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  • MATSUMOTO Midori
    Department of Microbial Genetics, Research Institute for Microbial Dieseases, Osaka University
  • ICHIKAWA Norio
    Institute of Biotechnology, Mitsui Toatsu Chemical Co. Ltd.
  • TANAKA Shigeo
    Mitsubishi-kasei Institute of Life Sciences
  • MORITA Takashi
    Department of Microbial Genetics, Research Institute for Microbial Dieseases, Osaka University
  • MATSUSHIRO Aizo
    Department of Microbial Genetics, Research Institute for Microbial Dieseases, Osaka University

書誌事項

タイトル別名
  • Molecular cloning of .PHI.80 adsorption-inhibiting cor gene.
  • ファイ80 ツイカ カンセン ソガイ イデンシ cor ノ クローニング エイ

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抄録

It was reported that φ80 lysogens did not adsorb superinfecting phage φ80 and a gene responsible to the phenomenon was named cor (Kozyrev et al., 1982). We found that E. coli containing φ80 genome in lytic cycle also began to show resistance to superinfecting φ80 after 5min of infection. We cloned the cor gene and determined its nucleotide sequence. A putative cor gene product should be 92 amino acids long and have three hydrophobic cores and one hydrophilic region, indicating a possibility of membrane binding protein. The product may interact with φ80 receptor on inner- or outer-membrane of E. coli cells and inhibit the adsorption of superinfecting φ80. The cor gene is supposed to be transcribed from a unique promoter located close to the gene.

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