DNA structure of the B chromosome of rye revealed by in situ hybridization using repetitive sequences.
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- TSUJIMOTO Hisashi
- Kihara Institute for Biological Research, Yokohama City University
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- NIWA Katsumasa
- Plant Germ-plasm Institute, Faculty of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- 反復DNA配列のin situハイブリダイゼーションによるライムギB染色体のDNA構造〔英文〕
- ハンプク DNA ハイレツ ノ in situ ハイブリダイゼーション ニ ヨ
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Abstract
To clarify the DNA structure of the B chromosome of rye, Secale cereale L., we carried out in situ hybridization using probes of two highly repetitive sequences (pSc74 and pSc119) of rye A chromosome, of total genomic DNAs from plants with (4 B) or without (0 B) B chromosomes, and probes of fractionated genomic DNA (1.14-kb and 1.28-kb sequences) from a 4 B plant. Clones pSc74 and pSc119 did not hybridize to the B chromosomes except at small interstitial sites. A probe consisting of the total DNA of a 0 B plant hybridized over the entire length of all the A and B chromosomes except for the telomeric C-band region of the long arm of the B chromosome. A probe of total DNA from a 4B plant produced signals only in the terminal C-band region after prehybridization with the 0 B plant DNA. A probe consisting of DraI-digested 1.14-kb fragments from a 4B plant hybridized to the telomeric region of the B chromosome, whereas one of 1.28-kb fragments did so to an interstitial region of the A chromosome. On the basis of the in situ hybridization, and the cytological and molecular biological results, we assume that the B chromosome in rye originated once in the distant past and was then perpetuated in the populations.<br>
Journal
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- The Japanese Journal of Genetics
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The Japanese Journal of Genetics 67 (3), 233-241, 1992
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282680421988352
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- NII Article ID
- 10006699293
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- NII Book ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
- http://id.crossref.org/issn/0021504X
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- NDL BIB ID
- 3786312
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed