Cytogenetical gene mapping by reciprocal translocation and tertiary trisomic analyses in rice (Oryza sativa L.).
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- Nonomura Ken-Ichi
- Faculty of Agriculture, Kyushu University
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- Yoshimura Atsushi
- Faculty of Agriculture, Kyushu University
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- Iwata Nobuo
- Faculty of Agriculture, Kyushu University
書誌事項
- タイトル別名
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- Cytogenetical gene mapping by reciproca
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The orientation of RFLP linkage map and the assignment of RFLP markers to the restricted chromosomal segment were determined on seven pachytene chromosomes (1, 3, 4, 5, 8, 9 and 12) of rice. A total of 24 interchange breakpoints were detected by pachytene analysis of reciprocal translocation (RT) heterozygotes derived from crosses between 12 RT lines and normal diploid varieties. Further, 19 strains of japonica-indica hybrid tertiary trisomics were developed and provided for RFLP gene dosage analysis, resulting in locating 32 breakpoints of the extra tertiary chromosomes within genetic linkage segments defined by RFLP markers. From these results, the orientation of RFLP linkage groups on pachytene chromosomes and the assignment of 113 RFLP markers and four cloned rice genes to chromosome arms were conducted, together with the delimitation of centromeres on 6 RFLP linkage groups (1, 3, 4, 8, 9 and 12). Meanwhile, 15 groups of RFLP markers were incorporated with the restricted segments of pachytene chromosome. Compared with RFLP map, the physical distance of a part of the long arm of chromosome 1 was 5.4 to 8.8 cM/μm at the pachytene stage.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 72 (1), 41-49, 1997
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詳細情報 詳細情報について
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- CRID
- 1390282680422202112
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- NII論文ID
- 130000061209
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- NII書誌ID
- AA11077421
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- COI
- 1:CAS:528:DyaK2sXktF2jsrw%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 4196968
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可