STUDIES ON SOMATIC CELL MUTATIONS

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  • BAN SADAYUKI
    Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University
  • SUZUKI FUMIO
    Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University
  • HORIKAWA MASAKATSU
    Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University

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Other Title
  • 体細胞突然変異の研究-1-培養されたチャイニーズハムスターhai細胞における放射線および化学物質による栄養要求性ならびに非要求性突然変異の誘発〔英文〕
  • タイサイボウ トツゼン ヘンイ ノ ケンキュウ 1 バイヨウサレタ チャイニー
  • I. RADIATION- AND CHEMICAL-INDUCTION OF NUTRITIONALLY DEFICIENT AND SUFFICIENT MUTANTS IN CHINESE HAMSTER <i>HAI</i> CELLS <i>IN VITRO</i>

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A forward mutation system using prototrophic CH-hai Cl 23 cells (Ala+, Asn+, Pro+, Asp+, Hyp+, Glu+) isolated from an original Chinese hamster hai cell line was found to be much more sensitive than a reverse mutation system using auxotrophic CH-hai Cl 3 cells (TdR-), for detecting the mutations induced by chemical carcinogens or mutagens (4-nitroquinoline 1-oxide, N-methyl-N'nitro-N-nitrosoguanidine, ethyl methane-sulfonate), as well as by X-rays and UV. It was found that the antimicrobial food additive, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylic acid amide (furylfuramide: FF or AF2) has highly mutagenic activity so far as it was tested by the forward mutations. The forward mutation system, from prototrophic CH-hai Cl 23 cells to auxotrophs, established in this study can be applied as a useful system for detecting environmental mutagens and for elucidating the mechanisms of somatic cell mutation.

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