The molecular basis for the alternative stable phenotype in a behavioral mutant of Paramecium tetraurelia.
-
- Matsuda Atsushi
- Institute of Biological Sciences, University of Tsukuba
-
- Takahashi Mihoko
- Institute of Biological Sciences, University of Tsukuba
Bibliographic Information
- Other Title
-
- molecular basis for the alternative stable phenotype in a behavioral mutant of Paramecium tetraurelia
Search this article
Abstract
In the sexual reproduction of Paramecium tetraurelia, the somatic nucleus (macronucleus) undergoes massive genomic rearrangement, including gene amplification and excision of internal eliminated sequences (IESs), in its normal developmental process. Strain d4-662, one of the pawn mutants, is a behavioral mutant of P. tetraurelia that carries a recessive allele of pwB662. The pwB gene in the macronucleus of the strain has an insertion of the IES because a base substitution within the IES prevents its excision during gene rearrangement. Cultures of this strain frequently contain cells reverting to the wild type in the behavioral phenotype. The mutant and revertant cells maintained stable clonal phenotypes under the various environmental conditions examined unless they underwent sexual reproduction. After sexual reproduction, both mutant and revertant produced 2.7-7.1% reverted progeny. A molecular analysis performed on the macronuclear DNA of the mutant and revertant of d4-662 showed that much less than 1% of the mutant IES was precisely excised at every sexual reproduction of the strain. Therefore, the alternative phenotype of strain d4-662 seems to be caused by an alternative excision of the mutant IES.
Journal
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 76 (5), 289-294, 2001
The Genetics Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282680422520192
-
- NII Article ID
- 10007643565
-
- NII Book ID
- AA11077421
-
- COI
- 1:CAS:528:DC%2BD38XovVKjsg%3D%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL BIB ID
- 5984571
-
- PubMed
- 11817644
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed