Viability of Escherichia coli cells under long-term cultivation in a rich nutrient medium.
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- Fukuda Tomoyuki
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Nakahigashi Kenji
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Inokuchi Hachiro
- Department of Biophysics, Graduate School of Science, Kyoto University
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Abstract
We investigated the viability of Escherichia coli cells during long-term cultivation in Brain Heart Infusion (BHI) medium and observed that the number of viable cells increased, then decreased, and increased again, in this medium, and finally the cells died out within about 10 days. This cell death may result from an increase in the pH of the medium. After repeated cultivation in BHI, bacterial cells that did not die out even under conditions of further cultivation were obtainable from cultures showing a stabilized viable count. We propose that long-term cultivation in BHI medium is a good system for studying growth phase-specific events in E. coli cells, because the total life-cycle of a population of E. coli, including exponential growth, stationary phase, and extinction, can be seen during a period of only about 10 days. Also, this system clearly allows detection of a phenotype that may not be detectable in orther commonly used media. Moreover, in this report, we show that mutants displaying the GASP (growth advantage in stationary phase) phenotype appear at high frequency under long-term cultivation conditions.
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 76 (5), 271-278, 2001
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282680422523392
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- NII Article ID
- 130000060840
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- NII Book ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD38XovVGqug%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 5984563
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- PubMed
- 11817642
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed