Transient Alpha-helical Structure during Folding of Src SH3 Domain at Subzero Temperatures
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- QIN Zhijie
- Department of Physics. Kansai Medical University Department of Chemistry and Biochemistry University of California at Santa Cruz
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- VYAS Sandip
- Department of Physics. Kansai Medical University
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- FINK Anthony L.
- Department of Physics. Kansai Medical University
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- LI Jinsong
- Department of Physics. Kansai Medical University
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- KIHARA Hiroshi
- Department of Physics. Kansai Medical University
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説明
Substantial questions remain about the process of protein folding, including whether transient intermediates exist with significantly different secondary structure than the final fold. The src SH3 domain is a highly beta-rich structure with five betastrands. We report here a far-UV circular dichroism investigation of the refolding of His-tagged Src SH3 at subzero temperatures. We observed a transient a-helix-rich intermediate, indicating that the early stages of protein folding can involve the formation of intermediates with very different structures from the final conformation.
収録刊行物
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- The Journal of Kansai Medical University
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The Journal of Kansai Medical University 58 (2-4), 163-169, 2006
The Medical Society of Kansai Medical University
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詳細情報 詳細情報について
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- CRID
- 1390282680435108864
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- NII論文ID
- 130004701405
- 110006473202
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- NII書誌ID
- AN00046712
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- NDL書誌ID
- 9315296
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- ISSN
- 00228400
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可