Histochemical Studies on Metachromatic Mucin Stain with Hematein

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  • Histochemical Studies on Metachromatic

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1. Metachromasia was ma n ifested in the capsular and interterritorial matrix of tracheal cartilage, the goblet cells of trachea, duodenum, jejunum and colon, the interelastic sapaces of aorta, and the stroma of cornea, when these specimens fixed in 1% CPC-10% neutr al formalin were stained with a solution which was prepared by dissoloving 50 mg of hematein and 50 mg of aluminum ammonium sulfate in 50 ml of 0.1 M acetate buffer at pH 3.6.<BR>2. On the basis of positive result s obtained by testicular hyaluronidase, Streptomyces hyaluronidase and neuraminidase digestions, it is suggested that the metachromatic substances are composed of chondroitin sulfate A and C which are present in the tracheal cartilage, sialic acid which is present in the goblet cells of trachea, duodenum and jejunum, the interelastic spaces of aorta, and the stroma of corn ea, and O-acylated sialic acid which is present in the colonic goblet cells.<BR>3. In view of the results obtaining with methylation and sap o nification treatment in addition to enzymatic digestion, it is suggested that hematein-lake combines with the sulfate group of chondroitin sulfate A and C, and carboxyl group of sialic acid. In addition, since the metachromasia is inhibited by addition of MgC12, it is presumed that the combination is due to ionic bonding.

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