Effects of Dietary Antioxidant Supplement for the Sidestream Smoke Exposure in Dogs

This study was performed to examine the effect of the sidestream smoke exposure and the effects of the dietary antioxidant supplement for the exposition on the oxidative stress in dogs. Six healthy dogs (2 males,4 females) were exposed by sidestream smoke as secondary tobacco smoke for 30 min in a small closed chamber. Blood samples were collected for the measurement of the oxidative stress before and 0,30,60,90 and 120 min after the exposition. The oxidative stress was evaluated by d-ROMs test (reactive oxygen metabolites test). The oxidative stress was significantly higher after the exposition than before. In the meanwhile, the oxidative stress didn't elevate in dogs fed the medical food which contains rich antioxidants (vitamin C: 34mg, vitamin E: 102mg/day). Next, same exposition was performed after the administration of vitamin C: 350mg, vitamin E: 100mg, carotene: 5.77mg and coenzyme Q₁₀: 10mg once a day for 2 weeks. The oxidative stress didn't elevate in dogs given these antioxidants. Moreover, the oxidative stress also didn't elevate in dogs after the 2-week administration of the multi vitamin-mineral food supplement prepared for dogs. The food supplement contains vitamin C: 0.132mg, vitamin E: 1.11mg, carotene: 0.225mg, coenzyme C₁₀: 3mg and catechin: 15mg for a dog weighing 10kg per day. The results suggested that secondary tobacco smoke exposure induced an oxidative stress not only in smoking pet owners but also in pets been together, and the increase of oxidative stress was suppressed by the administration of the antioxidants. It is supposed that daily intake of antioxidants prevented the elevation of oxidative stress.