Regional Differences in Surface Anionic Sites in the Rat Thyroid Follicle Cells Shown by Cationized Ferritin Labeling
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- NISHIYAMA FUMIAKI
- Department of Anatomy
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- TAKATA KUNIAKI
- Department of Anatomy
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- FUKUDA MINORU
- Laboratory for Electron Microscopy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo 181, Japan
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- HIRANO HIROSHI
- Department of Anatomy
書誌事項
- 公開日
- 1982
- DOI
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- 10.2535/ofaj1936.58.4-6_289
- 公開者
- Editorial Board of Okajimas Folia Anatomica Japonica
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説明
Cationized ferritin (CF) was used as a visual probe for surface anionic sites in the thyroid follicle epithelial cells. The rat thyroid glands were dissociated by enzyme digestion. The sheets of follicle cells maintained their structural polarity. When labeled after aldehyde fixation, CF was uniformly distributed over the entire cell surface in a thin layer. No regional difference in CF binding pattern was seen. When living cells were Iabeled at 0-4°C, CF was randomly scattered in a dispersed fashion on the apical surface, while the baso-lateral surface was heavily coated with a continuous thick layer of CF. When living cells were incubated with CF at 37°C, a small number of CF were found in clusters along the microvilli on the apical surface, while CF remained continuously and evenly on the baso-lateral surface similar to that of the cells labeled at 0-4°C. These regional differences in binding pattern of CF suggest differences in the properties between the apical and the baso-lateral membranes. It may contribute to maintain the structural and functional polarity in thyroid follicle epithelial cells.
収録刊行物
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- Okajimas Folia Anatomica Japonica
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Okajimas Folia Anatomica Japonica 58 (4-6), 289-303, 1982
Editorial Board of Okajimas Folia Anatomica Japonica