Development and characterization of 14 microsatellite markers for <i>Buergeria japonica</i> (Amphibia, Anura, Rhacophoridae)

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  • Komaki Shohei
    Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
  • Igawa Takeshi
    Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
  • Nozawa Masafumi
    Center for Information Biology, National Institute of Genetics Department of Genetics, SOKENDAI
  • Lin Si-Min
    Department of Life Science, National Taiwan Normal University
  • Oumi Shohei
    Section of Agriculture and Forest, Amami City Government
  • Sumida Masayuki
    Institute for Amphibian Biology, Graduate School of Science, Hiroshima University

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  • Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae)

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Abstract

Buergeria japonica is a common frog species distributed throughout almost all islands in Ryukyu Archipelago. Because of their exceptionally wide distribution and higher physiological tolerance comparing to the other anurans, their demographic history and formation of distribution are intrinsic topics in the herpetological fauna of Ryukyu. Microsatellite marker is ideal genetic marker for such studies at inter- and intra-population level. We therefore developed microsatellite markers of B. japonica utilizing Ion PGM™ sequencing. As a result of the screening, we developed a total of 14 polymorphic markers. To test availabilities of these markers, we genotyped four island populations. The total number of alleles and expected hetelozygosities per locus ranged from 4 to 21 and 0.00 to 0.864, respectively. The phylogenetic relationship among the four populations based on the genetic distances of these markers was congruent with general divergence pattern of amphibians and reptiles in Ryukyu area. These markers developed in this study are considered to be useful for future studies about phylogeography and demography of this species.

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