Individual chromosome assignment and chromosomal collinearity in Gossypium thurberi, G. trilobum and D subgenome of G. barbadense revealed by BAC-FISH

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  • Gan Yimei
    College of Plant Science and Technology, Huazhong Agricultural University State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Chen Dan
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Liu Fang
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Wang Chunying
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Li Shaohui
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Zhang Xiangdi
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Wang Yuhong
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science
  • Peng Renhai
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science Anyang Institute of Technology
  • Wang Kunbo
    State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Agricultural Science

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The experiment on individual chromosome assignments and chromosomal diversity was conducted using a multi-probe fluorescence in situ hybridization (FISH) system in D subgenome of tetraploid Gossypium barbadense (Db), G. thurberi (D1) and G. trilobum (D8), which the later two were the possible subgenome donors of tetraploid cottons. The FISH probes contained a set of bacterial artificial chromosome (BAC) clones specific to 13 individual chromosomes from D subgenome of G. hirsutum (Dh), a D genome centromere-specific BAC clone 150D24, 45S and 5S ribosomal DNA (rDNA) clones, respectively. All tested chromosome orientations were confirmed by the centromere-specific BAC probe. In D1 and D8, four 45S rDNA loci were found assigning at the end of the short arm of chromosomes 03, 07, 09 and 11, while one 5S rDNA locus was successfully marked at pericentromeric region of the short arm of chromosome 09. In Db, three 45S rDNA loci and two 5S rDNA loci were found out. Among them, two 45S rDNA loci were located at the terminal of the short arm of chromosomes Db07 and Db09, whilst one 5S rDNA locus was found situating near centromeric region of the short arm of chromosome Db09. The positions of the BAC clones specific to the 13 individual chromosomes from Dh were compared between D1, D8 and Db. The result showed the existence of chromosomal collinearity within D1 and D8, and as well between them and Db. The results will serve as a base for understanding chromosome structure of cotton and polyploidy evolution of cotton genome and will provide bio-information for assembling the sequences of finished and the on-going cotton whole genome sequencing projects.<br>

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