High-throughput evaluation of aryl hydrocarbon receptor-binding sites selected via chromatin immunoprecipitation-based screening in Hepa-1c1c7 cells stimulated with 2,3,7,8-tetrachlorodibenzo-p-dioxin

DOI Web Site Web Site 被引用文献4件 参考文献108件
  • Kinehara Masaki
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • Fukuda Itsuko
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University
  • Yoshida Ken-ichi
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University
  • Ashida Hitoshi
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University

この論文をさがす

説明

Upon binding to ligands such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an aryl hydrocarbon receptor (AhR) is activated to form a heterodimer with an aryl hydrocarbon receptor nuclear translocator (Arnt) and binds to DNA. It has been shown that the binding of AhR to DNA depends on the dioxin response element (DRE) and controls xenobiotic-response genes. AhR-binding DNA fragments from mouse hepatoma Hepa-1c1c7 cells stimulated with TCDD were once enriched in a chromatin immunoprecipitation (ChIP) DNA library and screened through a high-throughput southwestern chemistry-based enzyme-linked immunosorbent assay (SW-ELISA). After screening 1,700 fragments, the ChIP-SW-ELISA screening strategy allowed us to isolate 77 fragments tightly interacting with AhR in the presence of TCDD. Only 39 of the 77 fragments appeared to contain a typical DRE, indicating that in some cases the DRE was dispensable for AhR-binding, while 75 fragments were located within promoter-distal regions. Genomic mapping of the 77 fragments enabled us to estimate 121 potential AhR targets including known targets such as Cyp1A1 and Cyp1B1, but only a limited number exhibited an altered expression dependent on TCDD. This study revealed the fact that TCDD-activated AhR frequently binds to promoter-distal regions even without a DRE and is not always involved in transcriptional regulation, suggesting that within the genome DNA-binding of AhR could take place often in many regions without cis-regulatory elements and might not be a key determinant to establish its regulatory function.<br>

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (108)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ