Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101
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- Schenke Dirk
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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- Sasabe Michiko
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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- Toyoda Kazuhiro
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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- Inagaki Yoshi-shige
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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- Shiraishi Tomonori
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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- Ichinose Yuki
- Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University
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Here we report the genomic structure including the promoter sequence and coding region of NtPDR1 (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 78 (6), 409-418, 2003
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390282680449901312
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- NII論文ID
- 130000062105
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 6843140
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- PubMed
- 14973342
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可