Cloning and characterization of a novel radish protein kinase which is homologous to fungal cot-I like and animal Ndr protein kinases
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- Imai Takehiro
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Shimamura Satoshi
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Kurosaka Akira
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Yamagishi Hiroshi
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
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- Terachi Toru
- Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University
Bibliographic Information
- Other Title
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- Cloning and characterization of a novel radish protein kinase which is homologous to fungal cot-1 like and animal Ndr protein kinases
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Abstract
According to the similarity of the amino acid sequences in their catalytic domains, eukaryotic protein kinases have been classified into the five main groups: ‘AGC’, ‘CaMK’, ‘CMGC’, ‘PTK’ and ‘other’. The AGC group, represented by the cyclic nucleotide-dependent kinases (PKA and PKG), the calcium-phospholipid-dependent kinases (PKC) and the ribosomal S6 protein kinases, are poorly characterized in plants except for a few cases. In this study, in order to gain a better understanding of plant protein kinases in the AGC group, three cDNAs encoding novel protein kinases, RsNdr1 and RsNdr2a/b, were cloned from radish and characterized by molecular and biochemical methods.<br> The deduced amino acid sequences of RsNdr1 and RsNdr2a/b contained all 12 conserved catalytic subdomains which are characteristic of the eukaryotic Ser/Thr protein kinases. A cell lysate from E. coli overexpressing RsNdr1 fusion protein had protein kinase activity toward a conventional protein substrate (myelin basic protein), whereas that from E. coli harboring a fusion plasmid encoding kinase-dead RsNdr1 or RsNdr2 did not show any protein kinase activity. A phylogenetic tree for 17 protein kinases from various organisms showed that the RsNdrs are more closely related to the protein kinases in a particular subgroup of the ‘AGC’ (fungal cot1-like and animal Ndr kinases) than to the authentic ‘AGC’ protein kinases, such as PKA, PKC or ribosomal S6 kinase.<br>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 79 (5), 283-291, 2004
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282680450503168
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- NII Article ID
- 10014217490
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- NII Book ID
- AA11077421
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- ISSN
- 18805779
- 13417568
- http://id.crossref.org/issn/13417568
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- NDL BIB ID
- 7186239
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- PubMed
- 15599058
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed