Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa
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- Tanaka Shuuitsu
- Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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- Takayanagi Noriko
- Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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- Murasawa Kennichi
- Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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- Ishii Chizu
- Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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- Inoue Hirokazu
- Laboratory of Genetics, Department of Regulation-Biology, Faculty of Science, Saitama University
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Description
The un-17 mutant was originally isolated as an irreparable temperature-sensitive (ts) mutant in Neurospora crassa. Early experiments showed that cells of this mutant immediately stopped growing and died when the temperature of the culture was shifted from a permissive temperature (25°C) to non-permissive temperature (35°C). This ts phenotype is suppressed by addition of cycloheximide or in some conditions of growth repression. Even at the permissive temperature, it shows a female sterile phenotype and is deficient in production of exocellular superoxide dismutase SOD4 (EC 1.15.1.1). By searching for a DNA fragment that complements the ts phenotype of the un-17 mutant from a N. crassa genome library, we found the un-17 gene. The cloned un-17 gene encodes a homolog of the Saccharomyces cerevisiae poly(A) polymerase (PAP). The un-17 mutant had a one-base substitution mutation in the gene. The cloned un-17 genes from the wild-type strain and the un-17 mutant were introduced into both the un-17 mutant and wild-type strain. The un-17 mutant introduced by un-17 DNA from the wild-type strain showed recovery of both the ts and female sterile phenotypes. Moreover, the purified product derived from the wild-type strain showed PAP activity in vitro. These findings indicate that the un-17 mutant carries a ts mutation in the gene encoding PAP.<br>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 82 (6), 447-454, 2007
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390282680450791936
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- NII Article ID
- 10024395158
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- NII Book ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 9353588
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed