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- 内藤 克輔
- 金沢大学医学部泌尿器科学教室
書誌事項
- タイトル別名
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- FIBRINOLYTIC ACTIVITY OF IN VITRO CULTIVATED HUMAN UROLOGIC CANCEROUS TISSUES
- ソシキ バイヨウホウ ニヨル ヒニョウキ アクセイ シュヨウ ノ fibrin
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説明
In order to estimate fibrinolytic activity produced by human urologic malignant cells, surgical specimens from the malignant and normal tissues have been studied by a monolayer culture method. The fibrinolytic activity yielded into the supernatant medium and fibrin degradation products (FDP) resulted from coagulum lysis present in the system were determined by using a fibrin plate method and FDP assay Kit (Wellcome Reagents Ltd.), respectively. The localization of fibrinolytic activity in the cultivated cells was studied by a histochemical fibrin slide technique with a slight modification.<br>The results obtained were as follows:<br>1. An active growth of epithelial cells was observed in 11 renal cell carcinomas, a Wilms' tumor, an angioleiomyolipoma, 2 transitional cell carcinomas of the renal pelvis, and 4 transitional cell carcinomas of the bladder.<br>2. In the case of normal tissues, the active growth was observed in the cortex of 17 kidneys and the medulla of 4 kidneys. No active growth was observed in mucous membranes from a renal pelvis and 5 bladders.<br>3. Using the fibrin plate method, no fibrinolytic activity was detected in supernates from the renal cell carcinomas, the transitional cell carcinomas of the bladder, and HeLa cells.<br>4. The cortex and medulla released a potent plasminogen activator into the supernates; 0.20-25.36 and 1.88-5.00 CTA units/ml/1.0×104 cells, respectively. The plasminogen activator was identified immunologically as urokinase type.<br>5. No FDP was detected in cultures from a transitional cell carcinoma of the bladder and HeLa cells after 4 day cultivation, whereas, a considerable amount of FDP in cultures from the cortex of 4 kidneys and a renal cell carcinoma in the same cultivation period; the levels were 7.5-38.6 and 4.1.μg/ml/1.0×104 cells, respectively.<br>6. Using the fibrin slide technique, epithelial cells from the cortex of 4 kindeys in the same cultivation period showed a marked lysis of fibrin membranes in 30 minute-incubation time. The cells from a renal cell carcinoma showed less fibrinolytic activity. No lysis of fibrin membranes was observed in the cells from a transitional cell carcinoma of the bladder and HeLa cells in 180 minute-incubation time.<br>The physiologic and clinical significances of these findings are discussed with special reference to the combined use of urokinase and anticancer agents for urologic cancers.
収録刊行物
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- The Japanese Journal of Urology
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The Japanese Journal of Urology 68 (10), 909-923, 1977
社団法人 日本泌尿器科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680453581184
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- NII論文ID
- 130006933247
- 110003080702
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- NII書誌ID
- AN00196577
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- ISSN
- 18847110
- 00215287
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- NDL書誌ID
- 1914439
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- データソース種別
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- JaLC
- NDL
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