組織培養法による泌尿器悪性腫瘍の fibrinolysis の研究

DOI Web Site Web Site

書誌事項

タイトル別名
  • FIBRINOLYTIC ACTIVITY OF IN VITRO CULTIVATED HUMAN UROLOGIC CANCEROUS TISSUES
  • ソシキ バイヨウホウ ニヨル ヒニョウキ アクセイ シュヨウ ノ fibrin

この論文をさがす

抄録

In order to estimate fibrinolytic activity produced by human urologic malignant cells, surgical specimens from the malignant and normal tissues have been studied by a monolayer culture method. The fibrinolytic activity yielded into the supernatant medium and fibrin degradation products (FDP) resulted from coagulum lysis present in the system were determined by using a fibrin plate method and FDP assay Kit (Wellcome Reagents Ltd.), respectively. The localization of fibrinolytic activity in the cultivated cells was studied by a histochemical fibrin slide technique with a slight modification.<br>The results obtained were as follows:<br>1. An active growth of epithelial cells was observed in 11 renal cell carcinomas, a Wilms' tumor, an angioleiomyolipoma, 2 transitional cell carcinomas of the renal pelvis, and 4 transitional cell carcinomas of the bladder.<br>2. In the case of normal tissues, the active growth was observed in the cortex of 17 kidneys and the medulla of 4 kidneys. No active growth was observed in mucous membranes from a renal pelvis and 5 bladders.<br>3. Using the fibrin plate method, no fibrinolytic activity was detected in supernates from the renal cell carcinomas, the transitional cell carcinomas of the bladder, and HeLa cells.<br>4. The cortex and medulla released a potent plasminogen activator into the supernates; 0.20-25.36 and 1.88-5.00 CTA units/ml/1.0×104 cells, respectively. The plasminogen activator was identified immunologically as urokinase type.<br>5. No FDP was detected in cultures from a transitional cell carcinoma of the bladder and HeLa cells after 4 day cultivation, whereas, a considerable amount of FDP in cultures from the cortex of 4 kidneys and a renal cell carcinoma in the same cultivation period; the levels were 7.5-38.6 and 4.1.μg/ml/1.0×104 cells, respectively.<br>6. Using the fibrin slide technique, epithelial cells from the cortex of 4 kindeys in the same cultivation period showed a marked lysis of fibrin membranes in 30 minute-incubation time. The cells from a renal cell carcinoma showed less fibrinolytic activity. No lysis of fibrin membranes was observed in the cells from a transitional cell carcinoma of the bladder and HeLa cells in 180 minute-incubation time.<br>The physiologic and clinical significances of these findings are discussed with special reference to the combined use of urokinase and anticancer agents for urologic cancers.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ