男性性腺及び附屬性腺分泌機能に關する研究

Recently a various problems about male sexual organs and their accessory glands have been studied actively. Fructose test for the determination of fructose in semen, ability of sperm fructolysis and distribution of SH-group were studied by me for this purpose. The relationships between SH-group and fructolytic ability or sperm motility were submitted to the consideration and applicated to the clinical uses and animal experiments. It was found that SH-groups have a close relation to the secretory function of male sexual organ, particularily to the sperm, which may conduce to the diagnosis of the disorders of the male sexual system, selection of treatment and evaluation of therapeutic effect. Variation of fructose contents and SH-groups etc. in a various conditions enforcing some artifical stress was investigated clinically and the following results were obtained, (I adopted Roe's new-method for the determination of fructose, Huggins and Talalay's method for acid phosphatase, Flesch's method and amperometric titration for SH-groups, spectrography for inorganic ions, and Barrnett's method for the histochemical staining of SH-groups.)<br>1) Epididymal sperms may move in the isotonic solution simply, but in the 0.5% fructose-Ringer-phosphate-buffer solution, they may move more actively. Moreover the motility of the epididymal sperms may be activated by adding glutathion to them.<br>2) Glutathion accelerate sperm fructolytic action in aerobic and anaerobic conditions. Inhibition of Thiol' agent by SH compounds appears markedly in aerobic condition.<br>3) By histo-chemical and biochemical examination, it is noticed that SH-compounds may be secreted mainly in the epididymis and seminal vesicles.<br>4) Male sexual hormone may accelerate the secretory function of the accessory glands and promote sperm protection and maturity by means of stimulation of epididymal cells. Metabolic regulator of the epididymal sperms may also be stimulated by it and fructolytic action increases consequently i. e. sperms are activated remarkably.<br>5) As sperms start moving, the fructolytic action appears and SH-group in sperm decreases, while SS-group increases at the same time. SS-group in sperm mostly consists in the neck and middle piece of moving sperm which may be a motive center of the sperm.<br>6) In my present studies, fructose contents in seminal plasma were 3.0mg/cc in normal human being, 2.8mg/cc in case of neurasthenia sexualis, 2.9mg/cc in case of oligospermia, 3.5mg/cc in case of azoospermia, 1.6mg/cc in case of hemospermia and 2.9mg/cc in the punctured fluid from seminal vesicles.<br>7) SH contents in the semen were 0.84mg/dl in normal human being, 0.89mg/dl in case of neurasthenia sexualis, 0.63mg/dl in case of oligospermia, 0.49mg/dl in case of Azoospermia, 1.44mg/dl in case of hemospermia, 6.9mg/dl in the punctured fluid from seminal vesicles and 2.5mg/dl in the prostatic secretion.<br>8) By split ejaculate method, semen may be ejaculated in order of prostatic secretion, sperm and seminal secretion, but prostatic secretion may be ejaculated out of order. Fructose consists in the last half of the ejaculated semen and the sperm is directly proportional to SH-contents.<br>9) SH-group has some relation with sperm by the consecutive ejaculation. The sperm is mostly preserved in the amplar glands.<br>10) The contrast medium in the vasoepididymo-vesiculography has a spermicidal action by means of inhibition of SH-group.<br>11) In case of hemospermia, the movement of the sperms may be interrupted in early stage. It may be caused by the lack of fructose and SH group due to the secretory disturbance of the seminal vesicles. In addition, 18 kinds of cation in the semen were demonstrated by spectrography, so more metal ion contents in the semen of hemospermia may have a spermicidal action.<br>12) In case of vasectomy, the secretion of fructose in the seminal vesicles is not affected, but the secretion of the SH group decreases.