INTERMEDIATE FILAMENT CYTOKERATIN AND VIMENTIN POLYPEPTIDE PATTERN OF RENAL CELL CARCINOMA

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  • Suzuki Akira
    Department of Urology, Faculty of Medicine, The University of Tokyo

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  • 腎癌の骨格蛋白, 中間径フィラメントのサイトケラチン, ビメンチンの研究

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Intermediate filaments were considered the specific marker proteins which recognized the differences between normal and transformed cells. Recent studies have revealed that intermediate filament proteins are expressed in tissues of different embryologic origin in characteristric patterns.<br>Cytokeratins are a family of polypeptides of intermediate filaments in which diverse epithelia are expressed in different, yet specific, combinations. Vimentin type of intermediates is present in cells of mesenchymal origin.<br>We have studied the cytokeratins and vimentin in normal kidney tissue, in comparison with those in renal cell carcinoma, by two dimentional gel electrophoreses and immunofluorescence microscopy.<br>As for basic cytokeratin by NEPHGE procedure, tissues of renal medulla contain cytokeratin polypeptide nos. 1, 2, 3, 4, and 6.<br>By contrast, renal cell carsinomas contain only cytokeratin no. 4, which resemble those of cortex containing cytokeratin no. 1. and 4.<br>As for acidic cytokeratin by IEF procedure, similar cytokeratin pattern is observed in normal kidney tissue and renal cell carcinoma except defecting cytokeratin no. 12 in renal cell carcinoma.<br>Vimentin is also observed in all renal cell carcinoma.<br>When frozen sections are examined by immunofluorescence microsiopy, all epithelia of the kidney tissues and renal cell carcinomas are stained with monoclonal cytokeratin antibody.<br>By contrast, vimentin antibody is not stained in normal tissue, but in renal cell carcinoma.<br>From this point of view, it is plausible that renal cell carcinoma is not a mere adenocarcinoma, but has a nature of sarcoma.

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