CRITICAL ASSESSMENT OF THE RELIABILITY OF THINLAYER CYLINDER-PLATE METHOD FOR ANTIBIOTIC ASSAY, WITH SPECIAL REFERENCE TO COMPARISON WITH THE DOUBLE-LAYER METHOD

DOI Web Site

Bibliographic Information

Other Title
  • 抗生剤力価測定法としての薄層平板カップ法の測定精度とくに二層法との比較
  • コウセイザイ リキカ ソクテイホウ ト シテ ノ ハクソウ ヘイバン カップホウ ノ ソクテイ セイド トクニ ニソウホウ ト ノ ヒカク

Search this article

Description

The double-layer cylinder-plate method or so-called FDA cup method has been widely used for the assay of antibiotics. The basal layer of the assay plate in this system prevents irregularities of inhibition circles otherwise caused by uneven bottom surface of a glass Petri dish. Smooth-bottomed plastic dishes have recently replaced glass dishes and the time appears ripe now for exploring the possibility if this basal agar layer could be omitted without affecting the accuracy of ssay.<BR>Employing 4 serial two-fold dilutions of 4 antibiotics (benzylpenicillin, tetracycline, erythromycin and streptomycin) comparative studies were made between the conventional double-layer cylinder-plate method and the thin-layer cylinder-plate method with 5ml seed layer in a plastic Petri dish.<BR>The results obtained are summarized as follows:<BR>1) No statistically significant difference (P at 5% level) could be found in variations of measured values for each antibiotic between the two assay systems.<BR>2) Variations in values obtained by these 2 methods in quadruplicate gave standard errors to range 130-77% at maximum and 119-84% at minimum with the double-layer method, and 128-78% at maximum and 120-83% at minimum with the thin-layer method, respectively.<BR>The foregoing results indicate that the thin-layer system using a plastic Petri dish with smoothsurfaced bottom can be a reliable and simple assay system for antibiotic assay comparable to the conventional double-layer technique.

Journal

Details 詳細情報について

Report a problem

Back to top