Cryopreservation of <I>In Vitro</I> Fertilized Embryos from Transgenic Rat by Ultratrapid Freezing

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  • 超急速凍結法を用いたトランスジェニックラット由来前核期受精卵の凍結保存
  • チョウ キュウソク トウケツホウ オ モチイタ トランスジェニック ラット ユ

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Abstract

We cryopreserved pronuclear stage-embryos from the Transgenic rats produced by introducing the rat GH antisense transgene, by ultrarapid freezing. The embryos were obtained by fertilization in vitro with the sperm of homozygous males for the transgene. After thawing of a part of cryopreserved embryos (97 embryos), the 40 embryos (41%) were morphologically normal, and were transferred to oviducts of recipients. All four live young (10%) obtained (one male and three females) preserved the transgene and exhibited dwarfism. We also produced the homozygous transgenic rats after brother-sister mating of those young. These results demonstrated the possibility of the cryopreservation of embryos by ultrarapid freezing for sustaining transgenic rat line.

Journal

  • Experimental Animals

    Experimental Animals 43 (2), 247-250, 1994

    Japanese Association for Laboratory Animal Science

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