Tissue Culture Study on Mongolian Gerbil's (<I>Meriones unguiculatus</I>) Malignant Melanoma

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  • MORITOMO Tadaaki
    <I>Laboratory of Fish Pathology, College of Agriculture and Veterinary Medicine, Nihon University</I>
  • SAITO Hiroshi
    <I>Laboratory of Veterinary Clinical Pathology, College of Agriculture and Veterinary Medicine, Nihon University</I>
  • WATANABE Tasuku
    <I>Laboratory of Fish Pathology, College of Agriculture and Veterinary Medicine, Nihon University</I>
  • MOCHIZUKI Koshi
    <I>Laboratory of Biomedical Science, College of Agriculture and Veterinary Medicine, Nihon University</I>

Bibliographic Information

Other Title
  • スナネズミ悪性黒色腫の組織培養に関する研究
  • スナネズミ悪性黒色腫の組織培養に関する研究〔英文〕
  • スナネズミ アクセイ コクショクシュ ノ ソシキ バイヨウ ニ カンスル ケン

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Abstract

We tried to culture melanoma cells from a Mongolian gerbil's (Meriones unguiculatus) malignant tumor. In primary culture, most of cells have abundant melanin granules in their cytoplasm. Melanin granules decreased through 5 to 15 serial passages and disappeared after 15 th passage. The morphology of the cells varied from spindle to large polydendritic cells. Although typical melanin granules were not seen when the cells were stained by Masson-Fontana method, the cells were positive for DOPA reaction. Electron microscopically, most of the cells have well-developed Golgi apparatus and rough-surfaced endoplasmic reticulum with dilated cistern, and premelanosome-like granules were frequently observed in their cytoplasm.

Journal

  • Experimental Animals

    Experimental Animals 40 (3), 385-388, 1991

    Japanese Association for Laboratory Animal Science

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