グリオーマの免疫染色定量評価

DOI

書誌事項

タイトル別名
  • Quantitative evaluation of immunohistochemical analysis of glioma

この論文をさがす

説明

For neuropathological diagnosis of glial tumors, molecular diagnosis is also important as well as classical histological diagnosis. Key molecules for glial tumors include mutations in isocitrate dehydrogenase (IDH) 1and 2, 1p/19q loss of heterozygocity (LOH), p53 mutation, ATRX mutation, MGMT promoter methylation, Tert promoter methylation as well as Ki67. Immunohistochemistry is simple, robust and universal detection methods for these mokecules compared to genetic analysis. We applied automatedquantitative analysis for detection of Ki67 index, p53 mutation and MGMT promoter methylation using Gunma-LI that were developed for Ki67 detection of brain tumors. Automated count (trial method) and manual count as a golden standard) of 18 samples of glioma sections were compared. We found automated analysis with Gunma-LI is useful for quantitative detection of p53 mutation and MGMT promoter methylation as well as Ki67 index.This method might be applied for individual molecular analysis instead of genetic examination in multicenter clinical trial.

収録刊行物

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

  • CRID
    1390282680481823872
  • NII論文ID
    130005150139
  • DOI
    10.11452/neurooncology.23.1_14
  • ISSN
    21870551
    18800742
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • JaLC
    • CiNii Articles
    • KAKEN
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ