Simulated gastrointestinal environment affects sustainability of adhesion factors in <i>Lactobacillus reuteri</i> DSM 20016<sup>T</sup>

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  • Kobayashi Tatsuya
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture
  • Tsuji Akira
    Department of Brewing and Fermentation, Junior College of Tokyo University of Agriculture
  • Kajikawa Akinobu
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture

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Other Title
  • <i>Lactobacillus reuteri</i> DSM 20016<sup>T</sup> が持つ腸管粘膜定着因子の疑似的消化管環境における持続性
  • Simulated gastrointestinal environment affects sustainability of adhesion factors in Lactobacillus reuteri DSM 20016T

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Description

<p>　Many species/strains of Lactobacillus colonize in the gastrointestinal tracts of animals including human, and some of them are used as probiotics. Lactobacillus reuteri is frequently isolated from the gut of wide range of animals, and thus as a model of the intestinal microbiota, investigations on mechanism of their colonization are underway. While previous studies have described some L. reuteri adhesins, comparative analysis of those proteins have rarely been pursued. In the current study, L. reuteri DSM 20016T was recruited and its potential adhesion factors were evaluated. After L. reuteri DSM 20016T was exposed in simulated gastrointestinal conditions, the remaining adhesins were detected. As a result, the adhesins were relatively resistant to simulated gastric juice but were sensitive to simulated intestinal fluid. RT-qPCR was used to examine changes in the expression of the proteins, and results indicated that the expression of the adhesins tended to be promoted in comparison with their expression during normal culturing. In conclusion, the in vitro assay of this study suggested that cell-surface adhesins of L. reuteri could be damaged by digestive process but the bacterium may be capable of reproducing those proteins thereafter.</p>

Journal

  • Milk Science

    Milk Science 65 (3), 171-178, 2016

    Japanese Dairy Science Association

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