Effects of Theaflavin on Chemokine Production in TNF-α-stimulated Oral Epithelial Cells

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  • Yoshitaka HOSOKAWA
    Department of Conservative Dentistry, Tokushima University Graduate School of Biomedical Sciences
  • Ikuko HOSOKAWA
    Department of Conservative Dentistry, Tokushima University Graduate School of Biomedical Sciences
  • Kazumi OZAKI
    Department of Oral Health Care Promotion, Tokushima University Graduate School of Biomedical Sciences
  • Takashi MATSUO
    Department of Conservative Dentistry, Tokushima University Graduate School of Biomedical Sciences

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  • テアフラビンが口腔上皮細胞のケモカイン産生に与える影響の解析

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<p> Purpose: Theaflavin, a polyphenol substance extracted from black tea, has several beneficial effects such as anti-cancer, anti-inflammatory, and anti-oxidant effects. However, the effect of theaflavin on the progression of periodontal disease is still unknown. The aim of this study was to examine the effect of theaflavin on chemokine production in IL-27-stimulated human oral epithelial cells. We focused on CXCR3 ligands, CXCL9, CXCL10, and CXCL11 production in TR146.</p><p> Methods: Human oral epithelial cells, TR146 cells, were used in this study. We measured chemokine production in TR146 cells by ELISA. We used Western blot analysis to detect the phosphorylation levels of signal transduction molecules in TR146 cells.</p><p> Results: Theaflavin-3,3’-digallate (TFDG) decreased CXCL9, CXCL10, and CXCL11 production in IL-27-stimulated TR146 cells. Western blot analysis showed TFDG inhibited Akt, ERK, STAT1, and STAT3 phosphorylation in IL-27-treated TR146 cells.</p><p> Conclusion: TFDG suppressed chemokine production in IL-27-stimulated TR146 cells. These data suggest that theaflavin might have a beneficial effect against periodontitis.</p>

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