DAMAGE AND REPAIR OF DNA IN URINARY BLADDER EPITHELIUM OF RATS TREATED WITH N-BUTYL-N-(4-HYDROXYBUTYL) NITROSAMINE

DOI Web Site
  • TSUDA Hiroyuki
    First Department of Pathology, Nagoya City University Medical School
  • MIYATA Yukitada
    First Department of Pathology, Nagoya City University Medical School
  • HAGIWARA Akihiro
    First Department of Pathology, Nagoya City University Medical School
  • HASEGAWA Ryohei
    First Department of Pathology, Nagoya City University Medical School
  • SHIRAI Tomoyuki
    First Department of Pathology, Nagoya City University Medical School
  • ITO Nobuyuki
    First Department of Pathology, Nagoya City University Medical School

Bibliographic Information

Other Title
  • Damage and Repair of DNA in Urinary Bla

Search this article

Abstract

N-Butyl-N-(4-hydroxybutyl) nitrosamine (BBN), which selectively induced urinary bladder tumors in several experimental animal species, was found to cause damage of DNA in the bladder epithelium. Wistar strain rats were given 100mg/kg of BBN intravesically and killed after 2, 6, 12, 24, or 48hr. DNA damage was examined by measuring the change in sedimentation pattern in an alkaline sucrose gradient. Amount of DNA in each fraction was determined by fluorescence spectrophotometry. At 2hr, the sedimentation profile shifted from heavier (No. 15, control peak) to lighter (Nos. 2∼4) fractions, and the maximum effect appeared at 6hr as a single peak in the lighter fractions. At 12hr, damaged DNA was in the process of repair as two peaks were present, one light and one heavy. At 48hr, the sedimentation profile showed a single peak identical with that of controls indicating complete repair of DNA.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top