Preparation of collagen sponge by bubbling method and trial of cell seeding

DOI

Bibliographic Information

Other Title
  • 発泡法を用いたコラーゲンスポンジの調製と細胞播種の試み

Description

We introduced many air bubbles into 5% I-type collagen solution containing tiny amounts of chloroform by homogenizing. The bubbled solution was freezed at -35 degree centigrade for half day, freeze-dried for 1 day, and cross-linked at 130 degree centigrade for 6 hours using a vacuum oven. After ethylene oxide sterilization, we cultured rat osteoblasts UMR106 cells in the prepared sponges. After 1 day incubation, the cell visbility rates were relatively high at around 10%, indicating good cell-adhesion condition.

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Details 詳細情報について

  • CRID
    1390282680572823424
  • NII Article ID
    130006963192
  • DOI
    10.14832/gsjsdmd.2003s.0.105.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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