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Functinal Analysis of the Four PsbP Members Encoded by a Multigene Family in Nicotiana tabacum
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- Ishihara Seiko
- Graduate School of Biostudies, Kyoto Univ.
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- Yamamoto Yumiko
- Graduate School of Biostudies, Kyoto Univ.
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- Ifuku Kentaro
- Graduate School of Biostudies, Kyoto Univ.
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- Sato Fumihiko
- Graduate School of Biostudies, Kyoto Univ.
Bibliographic Information
- Other Title
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- 選択的RNAiによるタバコ<I>psb</I>P遺伝子ファミリーの機能解析
Description
PsbP is an extrinsic 23-kDa protein in the oxygen-evolving complex of PSII and reported to retain ions essential for the water-splitting reaction. In amphidiploids Nicotiana tabacum, PsbP is nuclear-encoded by a multigene family of four members, which could be divided into two groups by the sequence similarity (group-I: 1A and 5B; group-II: 2AF and 3F). All PsbP members are accumulated and group II comprises major component of PsbP in N. tabacum. To clarify the reason why N. tabacum has four PsbP members, group-specific PsbP-silenced plants were generated by RNAi. When compared to control plants, both group-specific PsbP-silenced plants showed marked decrease of PSII activity, while group-II-silenced plants showed more significant decrease of PSII activity. This result indicates that expressions of all PsbP members are required for the maximum PSII activity in N. tabacum. Further functional characterization of two PsbP groups is on-going.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2005 (0), 012-012, 2005
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680603565312
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- NII Article ID
- 130006986961
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed