Production of male-sterile <I>Arabidopsis</I> plants by using a transcriptional repression domain and tapetum-specific nuclear regulator <I>MS1</I>
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- Ito Takuya
- Plant Molecular Biology Laboratory, RIKEN
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- Shinozaki Kazuo
- Plant Molecular Biology Laboratory, RIKEN
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- Ohme-Takagi Masaru
- Gene Function Research Laboratory, AIST
Bibliographic Information
- Other Title
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- 転写抑制ドメインとタペート層特異的核内制御因子MS1を用いた雄性不稔シロイヌナズナの作出法
Abstract
We previously identified a male sterile Arabidopsis mutant, ms1-8, and isolated MS1 gene. MS1 was expressed specifically in tapetums and transiently during tetrad stage. The mutant did not accumulate cell wall of microspores, both the microspores and the tapetums gradually became vacuolated, and finally empty locules resulted. The MS1 protein was suggested to be a nuclear regulator.<br> We examined here whether a fusion protein of the MS1 and a strong transcriptional repressor SRDX functions as a repressor, and changes wild-type plants to sterile. The fusion gene repressed the pollen development, but the effect was incomplete. We will discuss further this novel technique to generate male sterile plants.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2005 (0), 673-673, 2005
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680603761408
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- NII Article ID
- 130006987169
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed