Circadian Bioluminescence Rhythm and KaiC Phosphorylation Rhythm of PCR-based Mutants of Clock Gene <I>kaiC</I> in <I>Synechococcus elongatus</I> PCC 7942

DOI

Bibliographic Information

Other Title
  • PCRエラーを利用したシアノバクテリアの時計遺伝子<I>kaiC</I> の突然変異体の表現型とKaiCリン酸化リズム

Abstract

Cyanobacteria are the simplest organisms known to exhibit circadian rhythms. Three clock proteins, KaiA, KaiB, and KaiC are essential elements of the circadian clock of cyanobacteria. We demonstrated that the oscillation of KaiC phosphorylation is the primary oscillation of cyanobacterial clock. Thus, kaiC mutants display a wide range of circadian phenotypes. To understand the biochemical functions of KaiC protein, we obtained 900 mutants with PCR-error based mutagenesis followed by a high throughput screening for rhythmic phenotypes with bioluminescence and analyzed their phenotype. While mutations mapped to various locations of KaiC sequence, two clusters of period-altering mutations were found. We analyzed a correlation between the mutation sites and the effects of temperature and light on the period length or phase of the bioluminescence rhythm. We will also discuss on relationships between phenotype of in vivo circadian rhythms and properties of KaiC phosphorylation rhythm of the mutants.

Journal

Details 詳細情報について

  • CRID
    1390282680603850752
  • NII Article ID
    130006987309
  • DOI
    10.14841/jspp.2006.0.599.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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