Circadian Bioluminescence Rhythm and KaiC Phosphorylation Rhythm of PCR-based Mutants of Clock Gene <I>kaiC</I> in <I>Synechococcus elongatus</I> PCC 7942
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- Murayama Yoriko
- Graduate School of Science, Nagoya Univ.
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- Imai Keiko
- Graduate School of Biological Science, NAIST
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- Ito Hiroshi
- Graduate School of Science, Nagoya Univ.
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- Oyama Tokitaka
- Graduate School of Science, Nagoya Univ. CREST/SORST, JST
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- Kondo Takao
- Graduate School of Science, Nagoya Univ. CREST/SORST, JST
Bibliographic Information
- Other Title
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- PCRエラーを利用したシアノバクテリアの時計遺伝子<I>kaiC</I> の突然変異体の表現型とKaiCリン酸化リズム
Abstract
Cyanobacteria are the simplest organisms known to exhibit circadian rhythms. Three clock proteins, KaiA, KaiB, and KaiC are essential elements of the circadian clock of cyanobacteria. We demonstrated that the oscillation of KaiC phosphorylation is the primary oscillation of cyanobacterial clock. Thus, kaiC mutants display a wide range of circadian phenotypes. To understand the biochemical functions of KaiC protein, we obtained 900 mutants with PCR-error based mutagenesis followed by a high throughput screening for rhythmic phenotypes with bioluminescence and analyzed their phenotype. While mutations mapped to various locations of KaiC sequence, two clusters of period-altering mutations were found. We analyzed a correlation between the mutation sites and the effects of temperature and light on the period length or phase of the bioluminescence rhythm. We will also discuss on relationships between phenotype of in vivo circadian rhythms and properties of KaiC phosphorylation rhythm of the mutants.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 599-599, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680603850752
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- NII Article ID
- 130006987309
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed