Isolation and characterization of genes involved in tolerance to environmental stresses by activation tagging

DOI

Bibliographic Information

Other Title
  • アクティベーションタギング法による環境ストレス耐性関連遺伝子の探索

Description

Activation T-DNA tagging produces dominant gain-of-function mutants caused by increased or ectopic expression of endogenous genes by insertion of an enhancers in plant genome. This approach allows discovery of novel genes in which recessive, loss-of-function mutants have no obvious phenotype. In this study, we screened a mutant with oxdative stress resistant from Arabidopsis activation-tagged lines and characterized the genes involved in the stress tolerance. Until now, we generated approximately 8,500-activation tag lines. Among 2,750 lines, two types of mutants (pqr-236 and pqr-242) were isolated, which were resistant to oxidative stress caused by the paraquat treatment at 3.0μM. The pqr-236 mutant showed paraquat-resistant in the T3 generation. T-DNA insertion sites in these mutants were determined by TAIL-PCR. Now, we are determining the transcript levels of genes near the T-DNA and evaluating the contribution of overexpression of the respective gene to the stress tolerance.

Journal

Details 詳細情報について

  • CRID
    1390282680604033792
  • NII Article ID
    130006987519
  • DOI
    10.14841/jspp.2004.0.805.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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