Analysis of Cytosolic Tetrapyrrole Binding Protein in <I>Arabidopsis thaliana</I>

DOI

Bibliographic Information

Other Title
  • シロイヌナズナの細胞質型テトラピロール結合タンパク質の解析

Abstract

In plant, hemes are distributed to various organelles. In addition, tetrapyrroles act as plastid-derived signals that regulate nuclear photosynthetic genes. However, mechanism of distribution of tetrapyrroles into organelles is poorly understood. In higher plants, tetrapyrroles are presumably distributed with tetrapyrrole binding proteins (TBP). Here, to clarify this mechanism, we characterized genes of Arabidopsis, which exhibited homology to animal p22HBP, a tetrapyrrole binding protein.<br> In Arabidopsis, 6 genes were identified as p22HBP homologs. When the gene with highest homology (At1g17100) was fused to GFP and expressed in onion, GFP fluorescence was detected in cytoplasm. Increase in absorption around 400 nm was observed, when heme was added to the recombinant protein with Kd 0.4 µM. The protein exhibited the affinity to protoporphyrin IX (Kd 0.4 µM). Reconstitution with apo-peroxidase showed that the protein binds heme reversibly. These results suggest that At1g17100 is cytosolic TBP, which carries tetrapyrroles in higher plants.

Journal

Details 詳細情報について

  • CRID
    1390282680604242816
  • NII Article ID
    130006987714
  • DOI
    10.14841/jspp.2006.0.426.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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