Analysis of expressed sequence tags from prothallia of <I>Adiantum capillus-veneris<I>
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- Yamauchi Daisuke
- Department of Life Science, Himeji Institute of Technology
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- Sutoh Keita
- Department of Biological Sciences, Tokyo Metropolitan Univ.
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- Kanegae Hiromi
- Department of Biological Sciences, Tokyo Metropolitan Univ.
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- Horiguchi Tatsuya
- RIKEN, Plant Science Center
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- Matsuoka Ken
- RIKEN, Plant Science Center
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- Fukuda Hiroo
- RIKEN, Plant Science Center
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- Wada Masamitsu
- Department of Biological Sciences, Tokyo Metropolitan Univ. National Institute for Basic Biology
Bibliographic Information
- Other Title
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- ホウライシダ前葉体で発現する遺伝子の解析
Abstract
Analyses of complete genome sequences of seed plants, Arabidopsis and rice were performed. In contrast, there is little knowledge about fern genomes. To understand the genetic information of a fern, <I>Adiantum capillus-veneris<I>, normalized cDNA library was constructed from prothallia grown under white light and analysis of expressed sequence tag (EST) was carried out. Approximately 10,000 clones were sequenced and clustering of these obtained sequences was performed. As a result, 7,132 non-redundant groups were generated. These groups were subjected to similarity search for identification of the putative function. Approximately 1,600 EST groups were found to be similar to sequences of genes registered in the public database. About 1,100 EST groups showed similarity to sequences of unknown function. The remaining EST groups showed no significant similarity and were classified as novel sequences.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2004 (0), 698-698, 2004
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680604524032
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- NII Article ID
- 130006988109
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed