Novel blue-light photoreceptors found in the Stramenopile alga, <i>Vaucheria</i>.
-
- Kataoka Hironao
- Graduate School of Life Sciences, Tohoku Univ.
-
- Takahashi Fumio
- Graduate School of Sciences, Tokyo Metropolitan Univ. Division of Biological Regulation and Photobiology, NIBB
-
- Yamagata Daisuke
- Graduate School of Life Sciences, Tohoku Univ.
-
- Kasahara Masahiro
- Gene Research Center, Tokyo Univ. of Agriculture and Technology
-
- Wada Masamitsu
- Graduate School of Sciences, Tokyo Metropolitan Univ. Division of Biological Regulation and Photobiology, NIBB
Bibliographic Information
- Other Title
-
- 黄色植物多核細胞フシナシミドロで見つかった新奇青色光受容体
Abstract
Coenocytic alga, Vaucheria (Stramenopile) exhibits phototropism to blue-light (BL). The alga also shows a photocytomorphogenetic response: a growth point is induced at the center of BL-irradiated region of the cell. For this response not only chloroplast- and nuclear accumulation, but also gene expression by the irradiated nuclei is indispensable. We have isolated cDNAs encoding two novel LOV-proteins from V. frigida by means of RT-PCR and 3'- and 5' RACE. To date it is still obscure whether Vauchria lacks phototropin. The two LOV-proteins, made of ca. 350 aa., resemble each other. At least one of them may serve as a BL-receptor, because its LOV domain, heterologously expressed in E. coli, bound with FMN. The both fusion proteins with GFP expressed in onion epidermis were located in nuclei. RNAi is used to elucidate what BL response is mediated by these photoreceptors.
Journal
-
- Plant and Cell Physiology Supplement
-
Plant and Cell Physiology Supplement 2005 (0), 251-251, 2005
The Japanese Society of Plant Physiologists
- Tweet
Details 詳細情報について
-
- CRID
- 1390282680604669696
-
- NII Article ID
- 130006988278
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed