Novel blue-light photoreceptors found in the Stramenopile alga, <i>Vaucheria</i>.

DOI
  • Kataoka Hironao
    Graduate School of Life Sciences, Tohoku Univ.
  • Takahashi Fumio
    Graduate School of Sciences, Tokyo Metropolitan Univ. Division of Biological Regulation and Photobiology, NIBB
  • Yamagata Daisuke
    Graduate School of Life Sciences, Tohoku Univ.
  • Kasahara Masahiro
    Gene Research Center, Tokyo Univ. of Agriculture and Technology
  • Wada Masamitsu
    Graduate School of Sciences, Tokyo Metropolitan Univ. Division of Biological Regulation and Photobiology, NIBB

Bibliographic Information

Other Title
  • 黄色植物多核細胞フシナシミドロで見つかった新奇青色光受容体

Abstract

Coenocytic alga, Vaucheria (Stramenopile) exhibits phototropism to blue-light (BL). The alga also shows a photocytomorphogenetic response: a growth point is induced at the center of BL-irradiated region of the cell. For this response not only chloroplast- and nuclear accumulation, but also gene expression by the irradiated nuclei is indispensable. We have isolated cDNAs encoding two novel LOV-proteins from V. frigida by means of RT-PCR and 3'- and 5' RACE. To date it is still obscure whether Vauchria lacks phototropin. The two LOV-proteins, made of ca. 350 aa., resemble each other. At least one of them may serve as a BL-receptor, because its LOV domain, heterologously expressed in E. coli, bound with FMN. The both fusion proteins with GFP expressed in onion epidermis were located in nuclei. RNAi is used to elucidate what BL response is mediated by these photoreceptors.

Journal

Details 詳細情報について

  • CRID
    1390282680604669696
  • NII Article ID
    130006988278
  • DOI
    10.14841/jspp.2005.0.251.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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