Identification of a vinyl reductase gene for chlorophyll synthesis in <I>Arabiopsis thalinana</I>


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  • クロロフィル合成酵素8-ビニルレダクターゼ遺伝子の同定と機能解析


Chlorophyll metabolism has been studied with various organisms and almost all of the chlorophyll biosynthetic genes have been identified in higher plants. However, only the gene for 3, 8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophyll synthesis has not been identified yet. <br>In this study, we isolated an Arabidopsis thaliana mutant that accumulated divinyl chlorophyll instead of monovinyl chlorophyll by ethyl-methanesulfonate mutagenesis. We identified the gene by Map-based cloning of this mutant. The mutant phenotype was complemented by the transformation with the wild-type gene. A recombinant protein encoded by the identified gene was expressed in E. coli and found to catalyze the conversion of divinyl chlorophyllide to monovinyl chlorophyllide, thereby demonstrating that the gene encodes a functional DVR. <br>Further, identification of the DVR gene helped understanding the evolution of Prochlorococcus marinus, a marine cyanobacteria that is unique in using divinyl chlorophylls.



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