Uncovering the effect of silent plant phenotypes from the combination of multivariate analysis and functional metabolic network analysis
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- Kusano Miyako
- PSC, RIKEN
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- Fukushima Atsushi
- PSC, RIKEN
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- Arita Masanori
- Graduate School of Frontier Sciences, The Univ. of Tokyo
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- Jonsson Par
- Umea plant science center (UPSC)
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- Moritz Thomas
- Umea plant science center (UPSC)
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- Kanaya Shigehiko
- Graduate school of information science, NAIST
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- Suzuki Hideyuki
- Kazusa DNA Research Institute
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- Shibata Daisuke
- Kazusa DNA Research Institute
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- Watanabe Mutsumi
- Graduate School of Pharmaceutical Sciences, Chiba Univ.
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- Noji Masaki
- Graduate School of Pharmaceutical Sciences, Chiba Univ.
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- Saito Kazuki
- PSC, RIKEN Graduate School of Pharmaceutical Sciences, Chiba Univ.
Bibliographic Information
- Other Title
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- GC-TOF/MSを用いたメタボロミクス方法論の確立-多変量解析と相関係数によるfunctional metabolic networks
Abstract
Silent phenotypes are genetically modified organisms that do not show apparent changes in morphology, yield, or growth rates when compared with parental lines under given physiological conditions. They undergo, however, subtle but systematic metabolic changes that are difficult to detect by targeted metabolite analysis. To analyze silent plant phenotypes, we used a double knock-out mutant of Serat genes (serat2;1 serat2;2); the mutant is known to exhibit relatively reduced amount of O-acetyl-L-serine, cysteine, and glutathione, which play major roles in the sulfur assimilation. In our metabolome analysis, 30 replicates of wild-type roots and their 30 mutants were analyzed using GC-TOF/MS. When multivariate analysis was applied to the non-processed GC/MS files (470 peaks in root; 514 peaks in leaf), the PLS-DA score separated the groups of wild-type clearly from the mutants. Lastly, significance of correlations was assessed considering the number of reaction steps that connect the metabolite pairs in the metabolic network.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 005-005, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605147392
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- NII Article ID
- 130006988779
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed