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Expression of <I>Salicornia europaea</I> Arabinogalactan Protein Enhances Salt Tolerance in <I>Escherichia coli</I>
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- Akatsuka Satoko
- Dept. Biotechnol., Tokyo Univ. of Agric. & Technol.
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- Yamada Akiyo
- Dept. Biotechnol., Tokyo Univ. of Agric. & Technol.
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- Tanaka Yoshiyuki
- National Institute of Agrobiological Sciences
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- Ozeki Yoshihiro
- Dept. Biotechnol., Tokyo Univ. of Agric. & Technol.
Bibliographic Information
- Other Title
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- 耐塩性向上活性を有するアッケシソウのアラビノガラクタンタンパク質
Description
Salicornia europaea, a member of the family Chenopodiaceae, is a highly salt-tolerant halophyte, which is distributed in the salt marsh. To analyze the mechanisms of salt tolerance at cellular level, suspension-cultured cell was established. The suspension-cultured cell can grow in Murashige-Skoog medium containing 1x10 -7 M of 2,4-dichlorophenoxyacetic acid, and 1x10 -6 M of 6-benzylaminopurine. In addition, this suspension-cultured cell could grow in the presence of 600 mM NaCl. To find the key genes for the salt-tolerance, functional screening was performed using Escherichia coli as the host organism. A transformant expressing fasciclin-like arabinogalactan protein (FLA) homologue revealed to have enhanced salt tolerance. The role of FLA in the salt tolerance was investigated. This research was supported in part by Industrial Research Grant Program in '03 from NEDO.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2005 (0), 550-550, 2005
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605205504
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- NII Article ID
- 130006988843
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed