Characterization of BmMre11 and Rad50 protein

DOI

Bibliographic Information

Other Title
  • カイコMre11、Rad50タンパク質の機能解析

Description

Cellular DNAs are exposed to a variety of exogenous and endgenous DNA damaging agents producing double-strand breaks (DSB). These DNA lesions have to be repaired as soon as possible, since the DSBs cause chromosomal deletions or fatal damage for cells. Eukaryotic cells have, at least, two major pathways to repair DSBs. One is HR (homologous recombination), another is NHEJ (non-homologous end-joining). MRE11 and RAD50 were reported to make a heterotrimer complex with NBS1, and play an important role in early process of HR and NHEJ. In this study, we have cloned the cDNAs encoding BmMRE11 and BmRAD50 from a silkworm testis, and their nucleotide sequences were determined. Then we recloned the Mre11 cDNA into a E. coli expression vector and attempted to purify the recombinant protein. At present, the enzymatic properties of BmMre11 are being chracterized using a partially purified protein. In order to examine the relationship between these genes and HR, we are trying to suppress Mre11 and Rad50 mRNAs by RNAi (RNA interference) in cultured silkworm cells.

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Keywords

Details 詳細情報について

  • CRID
    1390282680605363968
  • NII Article ID
    130006989050
  • DOI
    10.11416/jsss.jsss72.0.53.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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