Transcription Factors that Regulates Gene Expression in Sulfur Assimilation
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- Dansako Tomoko
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
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- Suzuki Hideyuki
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
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- Sakurai Nozomu
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
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- Aoki Koh
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
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- Saito Kazuki
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst. Graduate School of Pharmaceutical Sciences, Chiba Univ.
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- Shibata Daisuke
- The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
Bibliographic Information
- Other Title
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- 硫黄代謝経路の遺伝子発現制御に寄与する転写因子の探索
Abstract
Sulfur is an essential nutrient for growth of plants. Recently, a cis-acting element which responds to sulfur deficiency was identified in Arabidopsis (Maruyama-Nakashita et al. 2005). Our aim is to search transcription factors regulating sulfur assimilation. <br>Two public databases, ATTED (Arabidopsis thaliana trans-factor and cis-element prediction database; http://www.atted.bio.titech.ac.jp/) and AtGenExpress JPN (http://pfg.psc.riken.jp/AtGenExpress/index.html) were used to find transcription factors of which expression correlate with those of sulfur assimilation related genes and are induced under sulfur deficiency. Here, we investigated a Myb gene as a candidate. We over-expressed a full-length cDNA fragment of the gene in Arabidopsis suspension-cultured cell lines. Transcriptome analysis showed reduction of expression of a high affinity sulfate transporter gene. Furthermore, metabolite analyses using capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) showed reduction of contents of sulfur-related metabolites. The results suggest that the Myb transcription factor down-regulates sulfur assimilation.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2006 (0), 110-110, 2006
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390282680605563904
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- NII Article ID
- 130006989201
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed